Experimental NMR techniques for studies of biopolymers
نویسنده
چکیده
NMR technology has advanced significantly during the past year. Four-dimensional NMR spectroscopy is a feasible approach for unraveling the extremely crowded NOE spectra of medium-sized proteins. Alternatively, the use of selective deuteration also shows promise in this area. New three-dimensional NMR techniques can be used for accurate measurement of multiple-bond J couplings and improved methods are appearing for measurement of heteronuclear relaxation times, providing a more accurate characterization of internal protein dynamics.
منابع مشابه
Some aspects of the NMR chemical shift/structure correlation in the structural characterization of polymers and biopolymers
Studies on the NMR methodology for characterizing the structure of polymers and biopolymers based on the understanding of the NMR chemical shift/structure correlation using solution-state and solid-state NMR experiments, the development of the NMR chemical shift theory and their combination have been reviewed. Polymer Journal (2012) 44, 734–747; doi:10.1038/pj.2012.95; published online 13 June ...
متن کاملNuclear Magnetic Resonance Spectroscopy in Macromolecular Science
Today, virtually every experimental paper published in ‘‘Macromolecular Chemistry and Physics’’ includes characterization of the polymers under study by nuclear magnetic resonance (NMR) spectroscopy. In addition to monitoring the various synthetic steps, which eventually lead to the new polymers or supramolecular systems described, high-resolution NMR provides a number of important parameters, ...
متن کاملSensitivity enhancement in solid-state (13)C NMR of synthetic polymers and biopolymers by (1)H NMR detection with high-speed magic angle spinning.
Indirect detection of 13C and 15N nuclear magnetic resonance (NMR) spectra through 1H NMR signals offers large sensitivity advantages in studies of organic and biological molecules in solution and is almost universally employed.1-3 Although sensitivity enhancement by indirect detection was first demonstrated in NMR4-6 and nuclear quadrupole resonance7,8 of solids, direct detection has generally...
متن کاملApplicability of GPC, GC-MS and 13C NMR techniques and DFRC method in comparative structural studies of Lignin
In this research, the characteristics of CMP dioxane lignin (hardwood origin) and soda bagasse lignin (ethanol soluble fraction) and dioxane lignin of bagasse were studied using gel permeation chromatography, gas chromatography-mass spectrometry and 13C NMR spectroscopy and DFRC degradation procedure. The results indicated that the main structural units in lignins are phenyl propane skeletons b...
متن کاملConformational studies of Lewis X and Lewis A trisaccharides using NMR residual dipolar couplings.
The conformations of the histo-blood group carbohydrate antigens Lewis X (Le(x)) and Lewis A (Le(a)) were studied by NMR measurements of one-bond C-H residual dipolar couplings in partially oriented liquid crystal solutions. A strategy for rapid calculation of the difference between theoretical and experimental dipolar couplings of a large number of model structures generated by computer simula...
متن کامل